鲤春病毒血症病毒基质蛋白基因的原核表达及多克隆抗体的制备

根据鲤春病毒血症病毒(SVCV)基因组序列(GenBank:NC_002803),人工合成M基因开放阅读框序列,克隆至原核表达载体pET-32a(+),转化工程菌株BL21 (DE3),在0.1 mmol·L-1 IPTG、37℃下诱导5h,获得高效表达的M蛋白,但其主要以包涵体的形式存在,经超声破碎、包涵体纯化后,再...     

糠醛渣与蛭石混合基质对马铃薯试管苗生长发育的影响

试验观察了不同配比的蛭石粉、糠醛渣混合基质对马铃薯试管苗生长发育的影响。结果表明,蛭石粉和糠醛渣配比为25∶1时,马铃薯试管苗根长比对照增加0.9 cm,匍匐茎数比对照增加1.0个,匍匐茎膨大数相对优于其它处理,最有利于微型薯地下部的生长发育。     

思茅松嫩枝扦插育苗技术研究

从影响思茅松扦插生根的内因、外因和内外因相互作用等方面开展研究,应用对比分析、方差分析和多重比较寻找适合思茅松扦插的基质种类、植物生长调节剂浓度、穗条年龄和长度及扦插时间等,结果表明：思茅松基本属于愈伤组织生根型;8～10 cm长的穗条是嫩枝扦插较为合适的长度;扦插繁殖存在明显的年龄效应,1年生嫩枝的扦插生根率明显高于2年生母株上的嫩枝;嫩枝扦插以山地红壤较好。     

Advice in spite of great uncertainty: assessing and addressing bycatch of small fishes with limited data using Stellifer illecebrosus as a case study

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Interference elimination of PCB and its ramification in determining trace PBBs/PBDEs by GC/MS

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姑婆山国家森林公园景观格局变化分析

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